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Transient transfection of the PX459-sgRNA into the target cancer cell lines was performed using the Lipofectamine 3000 Transfection Reagent (Invitrogen) in 6-well plates (Corning Falcon) followed by puromycin selection (Gibco, 15 g/ml) for 3 days

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Patients presenting with deep-seated metabolic resistance, hypothyroidism, menopausal hormonal shifts, or chronic inflammatory states often struggle to lose weight even when adhering to strict caloric deficits

Neurotoxic and neuroprotective actions of catecholamines in cortical neurons