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Additionally, the role of estrogen in the modulation of glutamate, the primary excitatory neurotransmitter, has implications for synaptic plasticity and learning [26,30]

Cho KJ, Seo JM, Kim JH

The effects of BCAAs on fatigue may occur due to decreased transport of the amino acid tryptophan (which your body uses to make serotonin) into the brain, thus temporarily lowering serotonin levels[*]

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Following incubation, caspase 3 activity was measured using the fluorogenic substrate AC-DEVD-AMC as described before ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells