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Preparation of stock solution of n-acetyl l-cysteine 10 mg of n-acetyl l-cysteine was accurately weighed, transferred into a 10-ml volumetric flask and dissolved using a small amount of 0.1 N KOH, and then, the volume was made up using distilled water

The number after the arrow indicates the LOEL, dose for the effect reported

Total RNA was extracted using the TRIzol method, and the extracted RNA was reverse-transcribed into cDNA using the HiScript II Q RT SuperMix for qPCR (+ gDNA wiper) kit (Vazyme, China)

10.1002/9781119697473.ch17 190 RodrigoR.Gonzlez-MonteroJ.SotomayorC

Huffman DM, Farias Quipildor G, Mao K, Zhang X, Wan J, Apontes P, Cohen P, Barzilai N