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The NFT concentrations observed in the in vitro experiments for glutathione stimulation in this study can hence be considered to be representative or at least close of the concentrations that could be expected in vivo

However, new senescent cells continue to accumulate with ongoing aging, meaning the benefits would be expected to gradually diminish over time without retreatment

The pilot study and the main study took place during the months of April-June 2020, when the typical ambient temperature was 2325 C