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Topical treatments may take several weeks to show improvement, while laser treatments and chemical peels can offer more immediate results

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A programmed gradient of the mobile phase included a 3 min linear gradient from 100% (v/v) to 20% (v/v) methanol followed by another 3 min linear gradient from 20% (v/v) to 0% (v/v) methanol and hold at 0% (v/v) methanol for 6 min followed by a ramp from 0% (v/v) to 100% (v/v) methanol in 3 min and hold at 100% (v/v) methanol for 5 min