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At the time of the terminal experiments, hemodynamic measurement was performed by using a Mikro-Tip catheter (SPR-407, Millar Instruments, Houston, TX) to obtain left ventricular end-diastolic pressure (LVEDP), left ventricular end-systolic pressure (LVESP), heart rate, maximal slope of systolic pressure increment (+dP/dt) and diastolic pressure decrement (dP/dt)

We therefore examined whether this adaptive response is preserved in leptin-deficient ob/ob mice

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