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In summary, A549 cells (ATCC, CCL-185) were cultured and maintained at 37 C and 5% CO 2 in high-glucose (0.45% w/v) Dulbeccos Modified Eagle Medium (DMEM) with 10% (v/v) heat-inactivated fetal bovine serum, penicillin (50 U/mL), and streptomycin (50 g/mL)

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Mechanisms of Action (Preclinical) Angiogenesis: the combination supports new blood vessel formation via VEGF, FGF, and TGF- pathways, enhancing oxygenation and nutrient delivery to stressed tissues in research models

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