Under heat stress (39 C), spermatozoa exhibit mitochondrial dysfunction characterized by diminished membrane potential, impaired ATP synthesis, and a marked reduction in the activities of respiratory chain complexes I, III, IV, and V ( Figure 3b ) ( Figure 3b ) (75)
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This suggests that its mechanism of action may be unconventional, possibly involving the modulation of multiple downstream pathways or direct interaction with cellular components like growth factor receptors or cytoskeletal proteins