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For example, older people (because absorption declines with age) and those with gut-related conditions such as celiac disease and Crohns disease may struggle to absorb enough vitamin B12 to stave off symptoms of a deficiency, says Foxx

The role of endoplasmic reticulum stress in autoimmune-mediated beta-cell destruction in type 1 diabetes

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Subsequently, immerse the sections in eosin solution and stain at room temperature for 2 min, followed by a brief rinse with distilled water

AT samples were washed, connective tissue was removed and the fat was processed with scissors to obtain a homogenous mixture, that was digested with 500 units collagenase (Type II, Gibco) per gram of tissue in adipocyte isolation buffer (100 mM HEPES, 123 mM NaCl, 5 mM KCl, 1.3 mM CaCl2, 5 mM Glucose, 1% ZellShield, 4% BSA) for 45 min at 37 C