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After centrifugation at 30,000 g for 30 min, the supernatant fraction was loaded onto a nickelimmobilized metal ion affinity chromatography (NiIMAC) column (His GraviTrap, GE Healthcare)
They found that the 2D motility of HT-1080 fibrosarcoma cells, MDA-MB-231 breast cancer cells, and normal breast epithelial MCF-10 A cells is faster on fast- (~ 100 s) relative to slow-relaxing (~ 2,000 s), soft (2 kPa) substrates [143]
Patients should maintain adequate fluid intake based on individual needs, with attention to urine color (pale yellow indicates adequate hydration)
Young Innovator Association of CAS (2018325 (L.H.Z.))
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