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Coconut oil, olive oil, and cocoa/cacao butter are some of the most commonly used oils and butters when making herbal suppositories since they are all food-grade and easily melt when warmed or solidify when chilled

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Disclosure statement The authors do not have any commercial or other association that might pose a conflict of interest

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To test whether HNF4 confers sensitivity of liver tumors to methionine restriction in vivo, 5 10 6 siNeg and siHNF4 HepG2 cells were injected subcutaneously into each flank of female NU/J mice (#002019, Jackson Laboratory)