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The room was lit with regular room lighting (around 700 lux at the center of the box) and the floor of the open field was covered with the same type of bedding as used in their home cages

This means the B12 is not free to bind with other glycoproteins and move through the GI tract for absorption

Splitting a vial into two smaller pre-aliquoted vials is an option for large-volume reconstitutions, but requires sterile technique

It does not automatically represent the ideal target for every person