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Its replication was restricted to humans or experimentally infected chimpanzees, and efficient growth of the virus in cell culture failed until very recently

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The four constructs were transiently expressed using the Expi293 expression system, which resulted in high yields of purified monomeric protein fractions (180513 mg/L) that migrated according to their MW (73 kDa), and at the same rate as Tanzeum, on an SDS-PAGE gel, both under non-reducing and reducing conditions (Fig