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Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders MDCK cells were grown to 100% confluency on the coverslips in six-well plates and washed twice with PBS

In conscious swine, ipamorelin released GH with an ED50 of roughly 2.3 nmol/kg and an Emax of approximately 65 ng GH/mL plasma, the safety-and-specificity profile that has anchored every comparison study since

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