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GHK-Cu is found naturally in human plasma, saliva, and urine, and its concentration in plasma declines significantly with age, from approximately 200 ng/ml at age 20 to around 80 ng/ml by age 60

However, Tang et al

BALF was centrifuged to collect supernatants for cytokine and total protein content analysis, while cell pellets were used for differential cell counts via cytospin centrifuge and Hema-3 staining