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Procollagen I, IL-6 and TIMP-1 detection was carried out with a microplate reader (SpectraMax Plus 384 Microplate Reader, Molecular Devices, San Jos, CA, USA), while MMP-1 detection was performed with a spectrofluorometer (Varioskan Flash, Thermo Electron Corporation, Waltham, MA, USA)
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The difference varies with the age group, but among 65-to-74-year-olds the costs for smokers are as much as 40 percent higher among men and as much as 25 percent higher among women
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PMID 19188554
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