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Gibco, USA), non-essential amino acids, and penicillin/streptomycin, while SV-HUC culture medium was supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin

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FDA noted that use among high school students increased over the longer period from 2022 to 2025, but overall youth pouch use remained low and did not increase between 2024 and 2025