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The PCE of retatrutide were not weakened by the -adrenoceptor antagonist propranolol (1 M) but were blocked by 1 M carbachol, an agonist at M 2 -cholinoceptor, and this effect was reversed by 1 M atropine, a muscarinic receptor antagonist

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During the recruitment appointment and subsequent follow-up appointments at six and twelve months, the clinical status and mental health of all volunteers were evaluated, peripheral blood samples were collected, 45 plasma biomarkers (cytokines, chemokines, and growth factors) were assessed, and a comprehensive investigation was conducted using neuropsychological and ophthalmological tests, PET-CT, and magnetic resonance neuroimaging