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5-(Biotinamido)pentylamine (BP) incorporation assay Purified GST tag and human TG2 were incubated with BP (Pierce), 5 mM 1,4-dithiothreitol (DTT) (Sigma-Aldrich), and CaCl 2 (Sigma-Aldrich)

PC-3, DU-145, LNCaP and BPH cells were treated with ALCAR (1 or 10 mM) for 24 h

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They are considered for: Patients with type 2 diabetes: Individuals whose blood sugar remains high despite using first-line diabetic medications like metformin may benefit from adding a GLP-1 agonist

However, genetic or pharmacologic inhibition of RAS or its downstream signaling molecules (BRAF, MEK, and ERK) reversed the anticancer activity of erastin and RSL3 (Yang and Stockwell, 2008)