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Vectorial transport of the plant alkaloid berberine by double-transfected cells expressing the human organic cation transporter 1 (OCT1, SLC22A1) and the efflux pump MDR1 P-glycoprotein (ABCB1)

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Significance for Untargeted Metabolomics MoA Research This study illustrates the core value of metabolomics-based MoA characterisation: the activity difference between BSFL oil and its purified major fatty acid (C12:0) was only mechanistically interpretable through the lipid mediator metabolic landscape