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Single-point energies were calculated on the optimized geometries using the B97X-D hybrid functional 87 and the 6-311 + G(2d,p) basis set with ultrafine integration grids for the QM part and the ff14SB, gaff2 and TIP3P force fields for the MM part (protein, substrate and water, respectively) with electrostatic embedding

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One practical consequence of the triple mechanism: because three pathways are being engaged simultaneously, the total physiological load at any given milligram is higher than a single- or dual-agonist at the same number