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The eluted protein was concentrated by a size-exclusion spin column and exchanged into BRB80 buffer (80 mM HEPES, 1 mM MgCl 2 , and 1 mM EGTA, pH 6.8 by KOH) with 10% glycerol, 50 M ATP and 5 mM DTT

(GP-diagnosed, serum level below 150 pmol/L): Three injections over the first two weeks, then 1000mcg every two to three months

This is part of what makes peptides so appealing compared to typical pharmaceuticals: Many of the most common peptides used for medicinal purposes today (like for weight loss, diabetes, and hormone issues) are sequenced directly from peptides in our bodies

However, if you have a history of kidney issues or are on specific medications, you should consult your doctor first